Evaluation of reference genes for insect olfaction studies
نویسندگان
چکیده
منابع مشابه
Selection of suitable reference genes for real-time PCR studies of early developmental stages of sturgeons
In quantitative real-time PCR, the mRNA level can be quantified in relative terms based on the expression ratio of mRNAs of the target gene and an internal reference gene. Since, an internal standard should be expressed at a constant level among different tissues of an organism at all stages of development, and should be unaffected by the experimental treatment, the stability of different refer...
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Neuroethology utilizes a wide range of multidisciplinary approaches to decipher neural correlates of natural behaviors associated with an animal's ecological niche. By placing emphasis on comparative analyses of adaptive and evolutionary trends across species, a neuroethological perspective is uniquely suited to uncovering general organizational and biological principles that shape the function...
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BACKGROUND The Chinese white wax scale insect, Ericerus pela, is a well-known resource insect. The females and males are dramatically distinct at each developmental stage. We sought to identify suitable reference genes to use as internal controls in molecular research on E. plea. RESULTS geNorm, RefFinder and Normfinder analyses showed that ßTub-2 was the best reference gene throughout differ...
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ژورنال
عنوان ژورنال: Parasites & Vectors
سال: 2015
ISSN: 1756-3305
DOI: 10.1186/s13071-015-0862-x